Fig. 2

Characterization of CAQKERM@GsMTx4. A Schematic illustration of CAQKERM@GsMTx4 preparation. B Flow cytometry analysis showing Flag-tagged Blank and CAQK expression (Flag) in transfected and non-transfected HEK293T cells. C Quantification of the mean fluorescence intensity in B. D Confocal microscope pictures of CAQK showing up on the cell membrane of HEK293T cells that had Flag-tagged CAQK-expressing plasmid (CAQK) and Blank plasmid (Blank) added to them E Confocal microscopy images showing colocalization of CAQK (Alexa Fluor™ 488-conjugated) with markers for the endoplasmic reticulum, mitochondria, and lysosomes in CAQK-expressing HEK293T cells. F Standard curve for detecting GsMTx4 using a spectrophotometer. G GsMTx4 loading efficiency in different vesicles (n = 3). H Morphological characterization of EV’s, CAQK@EVs, CAQKERM@EVs, Blank@GsMTx4, CAQK@GsMTx4, and CAQKERM@GsMTx4 by TEM. Scale bar: 200 nm. I Size distribution analysis of EV’s, CAQK@EVs, CAQKERM@EVs, Blank@GsMTx4, CAQK@GsMTx4, and CAQKERM@GsMTx4 by nanoparticle tracking analysis. J SDS-PAGE and K Western blot analysis of membrane proteins