Fig. 1

Preparation and characterization of HSA@Pt. (A) TEM images of HSA@Pt. Scalar bar: 100 nm. (B) Hydrodynamic diameters of HSA@Pt measured by DLS. (C) Cumulative release of Pt from HSA@Pt in the presence of 10 mM GSH or PBS at 37 ℃, respectively. (D) Flow cytometric profiles and (E) the corresponding quantification of intracellular uptake of HSA@Cy5.5 for 0 h, 1 h, 4 h and 7 h, respectively, in A549 cells. (F) CLAS images of A549 cells treated with Cy5.5-labeled HSA@Pt at 0 h, 1 h, 4 h and 7 h, respectively. Scalar bar: 50 μm. (G) The confocal images of Cy5.5-labeled HSA@Pt endocytosed by H1299 3D spheroids. Scalar bar: 100 μm. Cell nuclear is strained by DAPI with blue fluorescence. The red fluorescence and green fluorescence come from Cy5.5 and cell skeleton stained with Actin, respectively. n = 3. Data are presented as mean ± SD. Statistical significances between every two groups were calculated vivo one-way ANOVA with Tukey’s multiple comparisons test. *p < 0.05, ** p < 0.01, *** p < 0.001.