Fig. 5
The effect of different combined solutions on mRNA/LNP. (A) Schematic of the LNP preparation method with different integrated solutions. (B) Transfection efficiency of mRNA/LNP in DC2.4 and AML12 cells. (C) Bioluminescent images and quantification of mice 6 h after i.v. (upper panel) and i.m. injection (lower panel) with Luc mRNA/LNP (5 µg mRNA per mouse) by the IVIS imaging system. (D) Quantitively analyzing the luciferase signal in mice after i.v. injection. Quantitively analyzing the luciferase signal at injection site (E), liver (F) and the ratio between liver and injection site (G) after i.m. injection. Data was presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001