Fig. 5

PGLNs alleviated LPS-induced ALI in mice, which may be related to the regulation of macrophage polarization. (A) Schematic diagram of LPS and PGLNs administration regimens in ALI mice. (B) Differences in body weight between the LPS group and LPS+PGLNs group (n = 5). (C) Macroscopic images of mouse lung tissues in each group. Unit: cm. (D) Protein concentration in the alveolar lavage fluid of mice detected by BCA (n = 4). (E) mRNA levels of IL-1β, IL-6, TNF-α, IL-10 and TGF-β1 in mouse lung tissues detected by RT-qPCR (n = 4). (F) H&E staining results of mouse lung sections. Scale: 200μm and 40μm. (G) Fluorescence expression of M1 pro-inflammatory macrophage marker CD86 and (H) M2 anti-inflammatory macrophage marker CD206 in mouse lung tissues detected by tissue immunofluorescence. Scale: 150 μm