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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Oral administration of Momordica charantia-derived extracellular vesicles alleviates ulcerative colitis through comprehensive renovation of the intestinal microenvironment

Fig. 5

The material basis of the efficacy of MCEVs. (A) KEGG annotated statistical charts of identified proteins in MCEVs. (B) Length distribution of miRNAs in MCEVs. (C) Relative quantification analysis of metabolites classes in MCEVs. (D) Relative quantification analysis of lipid classes in MCEVs. (E) Detection of nucleic acids of MCEVs after RNase A treatment by agarose gel electrophoresis. (F) Detection of protein of MCEVs after Proteinase K treatment by protein gel electrophoresis. (G) Schematic diagram of ultrafiltration method for removing excess impurities after enzymatic digestion. (H) qRT-PCR to evaluate the transcription level of inflammatory cytokines by RAW 264.7 macrophage cells after incubation with MCEVs of different treatments for 12 h and then LPS induced for 12 h. Data were shown as mean ± SD. n ≥ 3, *: P < 0.05, **: P < 0.01, ***: P < 0.001, ns: P > 0.05, indicating no significance

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Journal of Nanobiotechnology

ISSN: 1477-3155

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