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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Lactobacillus acidophilus extracellular vesicles-coated UiO-66-NH2@siRNA nanoparticles for ulcerative colitis targeted gene therapy and gut microbiota modulation

Fig. 1

Preparation and characterization of EVs@UiO-66-NH2@siRNA. (A) Representative TEM images (scale = 100 nm) of EVs, UiO-66-NH2, UiO-66-NH2@siRNA, and EVs@UiO-66-NH2@siRNA. (B) HAADF-STEM image of EVs@UiO-66-NH2@siRNA and elemental mapping of N, O, P, and Zr (Scale bar = 100 nm). (C) XRD spectra of UiO-66-NH2 and UiO-66-NH2@siRNA. (D) N2 adsorption-desorption isotherms of UiO-66-NH2 and UiO-66-NH2@siRNA. (E and F) Size and zeta potential of EVs, UiO-66-NH2, UiO-66-NH2@siRNA, and EVs@UiO-66-NH2@siRNA (n = 3). (G) Encapsulation efficiency of siRNA in EVs@UiO-66-NH2@siRNA (n = 3). (H) Time-dependent release of siRNA from EVs@UiO-66-NH2@siRNA at pH 3.0, pH 5.0, and pH 7.4 (n = 3). (I) Stability of UiO-66-NH2@siRNA and EVs@UiO-66-NH2@siRNA in serum (n = 3). (J and K) Degradation profiles over time of siRNA and EVs@UiO-66-NH2@siRNA in RNase and serum. (L) SDS-PAGE protein analysis of UiO-66-NH2, EVs, and EVs@UiO-66-NH2@siRNA (1: UiO-66-NH2, 2: EVs, 3: EVs@UiO-66-NH2@siRNA)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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