Fig. 5
Anti-inflammatory effects of EVs@UiO-66-NH2@siRNA in vivo. (A) Schematic representation of the experimental design. (B) Changes in body weight of each group of mice over 13 days (n = 5). (C) Changes in disease activity index of each group of mice over 13 days (n = 5). (D) Images of colon tissues. (E) Statistical analysis of colon lengths (n = 5). (F) Representative images of spleens. (G) Changes in spleen index (n = 5). (H-K) Quantitative RT-PCR analysis of relative mRNA expression levels of TNF-α, IL-6, IL-1β, and IL-10 in colon tissues (n = 5). (L-O) ELISA detection of TNF-α, IL-6, IL-1β, and IL-10 levels in colon tissues (n = 5). (P) Representative H&E-stained images of colon tissues at the end of treatment (scale bar = 20 μm). Data were presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001