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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Visualization of HSP70-regulated mild-photothermal therapy for synergistic tumor treatment: a precise space-time mild-temperature photothermal ablation strategy

Fig. 3

(a) NIR-II fluorescence images of 4T1 incubated with CA and CAC NPs (500 µg/mL) during different periods (1 and 2 h). (b) Cell viability of 4T1 cells after treated with different groups (PBS, PBS + Laser, CAC, CAC + Laser). (c) Cytotoxicity evaluation of CAC NPs (500 µg/mL) in 4T1 cells under different conditions. (d) Calcein AM/PI double staining in 4T1 cells cultured with different conditions: PBS, PBS + Laser, CAC, CAC + Laser. Concentration (CAC NPs) = 500 µg/mL. (e) The growth curves of 4T1 cells were subjected to different treatments at 500 µg/mL. (f) Colony formation assay of 4T1 cells with different treatments. (g) Western blot assays of 4T1 cells to detect the expression of the apoptotic-related proteins after different treatments. Scale bar: 50 μm

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