Fig. 4
From: Streptococcus mutans-derived extracellular vesicles promote skin wound healing via tRNA cargo
Effect of Sm EVs on EGFR activation. (A) Two different types of organoids were treated with 107 particles/mL of Sm EVs for 3 days. EGFR phosphorylation was assessed using Western blot analysis. (B) Organoids were pretreated with EGFR inhibitors, gefitinib (1.1 µM) or afatinib (500 nM), followed by Sm EV treatment for 3 days. EGFR phosphorylation (Tyr 1173) was assessed using Western blot analysis. (C) Morphological changes in organoids treated under the same conditions were monitored for 7 days using phase-contrast microscopy. (D) Organoids were pretreated with control siRNA or siTLR3 for 24 h, followed by treatment with Sm EVs for 7 days. EGFR phosphorylation (Tyr 1173) was assessed using Western blot analysis.