Fig. 6
From: Streptococcus mutans-derived extracellular vesicles promote skin wound healing via tRNA cargo
Effect of Sm EV-derived tRNA oligos on primary oral epithelial cell migration. (A) A flow diagram illustrating the preparation of Ec EVs loaded with oligos synthesized from tRNA sequences derived from Sm EVs. Images were created with BioRender.com. (B) For the scratch wound-healing assay, confluent monolayers of primary oral epithelial cells in a 12-well plates were scratched with a pipette tip to create a wound. Cells were then cultured in the presence of Ec EVs with or without Oligos 1 or 3 for 5 days, and wound closure was measured over time. (C) For the Transwell migration assay, primary oral epithelial cells were treated with Ec EVs carrying specific oligos for 24 h. Migrated cells were stained using 0.2% crystal violet in 10% ethanol and counted under a phase-contrast microscope. **p < 0.01