Fig. 6

(a) Schematic diagram of the schedule of in vivo anticancer treatment. (b) Body weight changes. (c) Individual tumor growth curves of 4T1 nude mice treated with different groups (n = 5). (d) Tumor inhibition rate in mice after different treatments (n = 5). (e) H&E, (f) Ki-67, (g) TUNEL staining images of tumor slices from tumor-bearing mice treated with various treatments. (h) cleaved Caspase 1, (j) N-GSDMD staining images and (i, k) the corresponding fluorescence semi-quantitative results of tumor slices from the tumor-bearing mice in different treatment groups. Statistical analyses were performed using one-way ANOVA. Data are expressed as mean ± standard deviation (SD), where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s: differences were not statistically significant