Fig. 3
From: Synphilin-1 regulates mechanotransduction in rigidity sensing through interaction with zyxin
Synphilin-1 overexpression induced the loss of cellular rigidity sensing on substrates with different stiffness. A Actin staining images of cells on substrates with different stiffness (scale bar: 20 μm). B–D Spreading area of cells on substrates with different stiffness in HEK293 (B), Synph-293 (C), and Synph-293-KD cells (D) (n = 50). E Focal adhesion of cells on substrates with different stiffness (scale bar: 20 μm). F–H Total area of focal adhesions of cells on substrates with different stiffness in HEK293 (F), Synph-293 (G), and Synph-293-KD cells (H) (n = 50). The inner solid line and cross sign indicate the median and mean values, respectively. Statistical significance was determined using a one-way ANOVA followed by Tukey’s HSD (B, F, G) and Games-Howell (C, D, H) comparison test