Fig. 4
(A) Confocal fluorescence images of cellular Fe2+ content in KYSE70 cells. Scale bar: 50 μm. (B) TEM images of mitochondrial morphological changes in KYSE70 cells. Scale bar: 400 nm. (C) Fluorescence images of ROS generation and effect on mitochondrial membrane potential in KYSE70 cells detected by DCFH-DA and (D) JC-1 staining with various treatments. Scale bar: 100 μm. (E) Fluorescence images of LPO in KYSE70 cells with various treatments. Scale bar: 50 μm. (F) MDA content of KYSE70 cells after various treatments