Fig. 6

In vitro anti-cancer activity of Au-Pt@ur NPs. (a-b) Concentration-dependent cytotoxicity of Au NPs or Au-Pt@ur NPs on T24 cells or 253 J cells for 12 h-incubation, illustrated by the CCK-8 assay. (c-e) Concentration-dependent cytotoxicity of Au-Pt@ur NPs + U (+ L), Au-Pt@ur NPs + H (+ L), Au-Pt@ur NPs + H + U (+ L) in T24 cells for 12 h-incubation, illustrated by the CCK-8 assay. (f) Cell apoptosis effect, illustrated by the flow cytometry analysis from the Annexin V-FITC-fluorescence channel and PI- fluorescence channel. (g) Averaged apoptotic rate of T24 cells for 12 h-incubation, illustrated by the flow cytometry analysis assay. (h) Intracellular ROS generation, illustrated by the flow cytometry analysis from the Annexin V-FITC-fluorescence channel. (i) Relative ROS level of T24 cells for 12 h-incubation