Fig. 2
Inhibiting Pg secreting OMVs rescues bone regeneration in Pg microbiota imbalance. A The growth curve of Pg under vehicle and GW4869 treatment. B BCA analysis showed that the protein contents of OMVs were reduced by GW4869. C NTA analysis indicated that the particle number of OMVs decreased remarkably in the medium after being treated with GW4869. D A schematic illustration of tooth extraction under periodontitis microenvironment model established by Pg or GW4869-treated Pg. E Representative micro-CT images of extraction sockets in the control group, Pg group and GW4869-treated Pg Group. F The bone regeneration of the extraction sockets was determined by micro-CT analysis of BV/TV, Th.N, and Th.Tb (control group: n = 6, Pg group: n = 6, GW4869-treated Pg Group: n = 6). G The new bone formation was determined by H&E staining (scale bar = 400 μm). H The collagen fiber deposition was evaluated by Masson staining of extraction sockets (scale bar = 400 μm)