Fig. 5

Pg-derived OMVs activate ferroptosis in BMSCs. A RNA sequence analysis of differentially expressed genes displayed in volcano plot. B KEGG pathway enrichment analysis between the control group and OMVs group. C The ACSL4, TFRC, PTGS2, and GPX4 expression levels were measured by qRT-PCR. D Western blotting results of ACSL4, TFRC, PTGS2, and GPX4, Full-length blots are displayed in supplementary Fig. 5. E Quantitative results of western blotting. F Transmission electron microscopy showed that mitochondria were deformed after Pg-derived OMVs treatment (scale bar = 500 nm). G Results of FerroOrange staining showed that Iron overload in Pg-derived OMVs treated cells (scale bar = 50 μm). H MDA assay results indicated that malondialdehyde was significantly increased after Pg-derived OMVs treatment. I immunofluorescence colocalization results showed that, in Pg-derived OMVs treated extraction socket, the expression of PTSG2 was dramatically increased in stro-1 positive BMSCs in vivo (scale bar = 50 μm)