Fig. 6

Inhibiting ferroptosis reverses the negative effect of Pg-derived OMVs on osteogenic differentiation of BMSCs. A FerroOrange staining revealed that the OMVs-induced increase in intracellular iron overloading was strongly blocked by Fer-1 (scale bar = 40 μm). B TEM results revealed that Pg-derived OMVs caused mitochondrial vacuolation and deformation, but mitochondrial vacuolation and deformation can be rescued by Fer-1 (scale bar = 500 nm). C The results of MDA assay. D qRT-PCR analysis of ALP, OCN, and BMP2. E, F Western blotting results of ALP, OCN, and BMP2. Full-length blots are displayed in supplementary Fig. 7. G Alizarin Red S staining results (scale bar = 250 μm). H The quantitative results of Alizarin Red S staining. I ALP activity staining results revealed the ALP activity of BMSCs was inhibited by Pg-derived OMVs, but the decline tendency was reversed by Fer-1 (scale bar = 250 μm)