Fig. 8

Pg-derived OMVs regulate BMSCs ferroptosis via the Hippo-YAP pathway. A Heatmap of RNA-sequencing analysis. B TF-Target Finder was used to predict and find the possible upstream transcription factor of ACSL4, TFRC, and PTGS2, respectively (based on ChIP Atlas database and GTRD database). The Venn diagram showed the intersection of predicted transcription factors of ACSL4, TFRC, and PTGS2. C KEGG pathway enrichment analysis. D The immunofluorescence results of YAP in the nucleus (scale bar = 30 μm). E, F Western blotting results of YAP protein and p-YAP protein. G The immunofluorescence results of YAP and stro-1 in the sockets (scale bar = 50 μm). H Western blotting results of YAP expression in the nucleus. I qRT-PCR results of TFRC, ACSL4, and PTGS2. J The protein expression levels of TFRC, ACSL4, and PTGS2 were measured by western blotting. Full-length blots are presented in supplementary Fig. 9